Genes up-regulated in NESCs encoded transcription factors involved in neural system development, such as PAX6, POU3F2, MEIS1, RFX4 [37, 39, 40], cell proliferation and suppression of terminal differentiation, such as TFAP2B (AP2beta) [41, 42], and an adhesive junction-associated protein of the armadillo/beta-catenin superfamily involved in brain development and several neurological disorders, i.e., CTNND2 [43, 44]. This evidence concerns the gene RFX4 and nervous system disorder.