Using this approach, a cytotoxic tumor microenvironment was associated with higher absolute numbers of CD68+pSTAT1+ (median 73 cells/mm2 for CD8+/FOXP3+ ratio >1.5 vs. median 12 cells/mm2 for CD8+/FOXP3+ ratio <0.75; P = 0.025, Mann-Whitney) and CD163+pSTAT1+ macrophages (median 26 cells/mm2 for CD8+/FOXP3+ ratio >1.5 vs. median 1 cell/mm2 for CD8+/FOXP3 ratio <0.75; P< 0.0005, Mann-Whitney). The gene discussed is FOXP3; the disease is neoplasm.