Furthermore, we also found that TMEM100 mRNA expression in the HCC cell line HepG2 and SMMC-7721, which with high metastatic and proliferative potential, was very low (0.001 and 0.002), while in HCCLM3 and MHCC97L, which with lower metastatic and proliferative potential, TMEM100 mRNA expression was relatively high (0.40 and 0.17). Here, TMEM100 is linked to hepatocellular carcinoma.