Mouse MC-38 colon adenocarcinoma cells were exposed to normoxia (21% oxygen) or hypoxia (0.2% oxygen) for 8 to 72 hours using conventional two-dimensional (2D) dish culture, and mRNA levels of HIF-1α and HIF-2α were determined by RT-qPCR. The gene discussed is EPAS1; the disease is colon adenocarcinoma.