The relatively lesser effect of the Cx43-T154A mutant in inhibiting cel-miR-67 transfer to glioma cells is consistent with its less robust effect on inhibition of gap junction coupling, relative to 18α-GA (Figure 1D, 1F, Supplementary Figure S2), likely due to the fact that wild type Cx43 is also being expressed in these cells. Here, GJA1 is linked to central nervous system cancer.