Furthermore, by analysis of the miRNA profile change, we identified two specific miRNAs, miR-5096 and miR-4519, which were increased in astrocytes after co-culture with glioma cells, and these increases were blocked when gap junction function was inhibited using three different methods (non-contact co-culture, gap junction blocker 18α-GA and Cx43 dominant negative mutant T154A). The gene discussed is GJA1; the disease is central nervous system cancer.