Although certain micrographs from our previous study showed cells with an accumulation of fluorescent signal for CD133 in the nucleus (6), we assumed that this finding was an artifact resulting from the use of a rabbit polyclonal antibody against CD133, (which was the only anti-CD133 antibody available at the time), although we had never detected similar nuclear positivity for CD133 in osteosarcoma or glioblastoma cell lines using the same antibody (5,18). This evidence concerns the gene PROM1 and osteosarcoma.