ERVW-1 and multiple sclerosis: A similar approach was demonstrated by Antony et al., where authors employed a real-time PCR approach using MRSV or syncytin-1-selective oligonucleotide primers and fluorescent, intercalating SYBR Green I dye which allowed the quantitative analysis of gene expression in brain tissue samples, peripheral blood leukocytes (PBLs) and monocyte-derived macrophages (MDM) from multiple sclerosis (MS) and non-MS patients as well as in an astrocytic U373 cell line.