Conversely, Type 1 regulatory T (Tr1) cells, which co-express CD223 and CD49b (Fig 3A, right panel), were slightly increased in 4x compared to 1x DEC as a proportion (Fig 3B, p<0.05), but the difference in the total number of Tr1 cells between the infection groups did not reach statistical significance (Fig 3C, p>0.05). This evidence concerns the gene LAG3 and infection.