Given the distinct cell-type specificity of expression of FRA and FRB, and the role of infiltrating or tumor associated inflammatory cells on the growth and metastasis of tumors, we sought to examine tumor and immune cell expression of both FRA and FRB in the context of gynecologic tissues, in particular EOC, using RNAscope, a novel, semi-quantitative, nucleic acid in situ hybridization (ISH) tool that allows for single molecule signal amplification and background suppression of individual cells [33] to evaluate, in architectural context, the expression patterns of FOLR1 and FOLR2. Here, FOLR2 is linked to neoplasm.