To evaluate the effect of FASN on cellular respiration, oxygen consumption rate (OCR) was measured in HCT116 and HT29 CRC cell lines with stable knockdown of FASN and in SW480 cells with stable overexpression of FASN using the Seahorse XF Extracellular Flux Analyzer (FASN expression in CRC cell lines is shown in Supplemental Figure 1). The gene discussed is FASN; the disease is colorectal carcinoma.