To test whether changes in the level of FASN expression and bioenergetic status of CRC cells correspond to changes in major energy-sensing pathways, HCT116 cells with stable knockdown of FASN and SW480 cells with stable overexpression of FASN were cultured in matrix-attached or matrix-detached conditions for 48 h prior to western blot analysis. Here, FASN is linked to colorectal carcinoma.