In fact, profilers based on the expression of a higher number of genes (CD3Z, CD8, CXCL9, CXCL13 and IGHM) and consisting of 2, 3 or 4 clusters did not show marked gene expression variability between tumour groups, did not improve separation of clusters nor did they exhibit prognostic significance for patient outcome (S1–S3 Figs). This evidence concerns the gene IGHM and neoplasm.