Importantly, western blot and real-time quantitative RT-PCR indicated that ectopic expression or knockdown of NFκB-p65 decreased and increased the expression of HNF4α, respectively, and prevented the gastric cancer cells from ITLN1-mediated changes in HNF4α expression (Figure 2D, Figure 2E, Figure S3C, Figure S3D, and Figure S3E). This evidence concerns the gene NFKB1 and gastric cancer.