Preoperative assessment of lymph node involvement represents a critical step for determining the extent of surgery in patients with endometrial carcinoma, especially in patients with clinical stage I. Interestingly, the mean value of CA125 in the 6.4% patients was significantly higher than those with FIGO stage I. In the current study, the CA125 serum level of 25 U/mL was the best cutoff to determine the lymph node metastasis without influence of adnexal involvement, distant metastasis and positive peritoneal cytology. Here, MUC16 is linked to metastatic malignant neoplasm in the lymph nodes.