MUC16 and metastatic malignant neoplasm in the lymph nodes: When we evaluated the best cutoff level of lymph node metastasis factors using a ROC curve in patients with clinical stage I, the CA125 serum level of 25 U/mL was best, with 75.4% of sensitivity, 73.7% of specificity, 83.1% of false positive rate, 2.3% of false negative rate (Figure 2A).