When we evaluated the best cutoff level of lymph node metastasis factors using ROC curve in all cases, the CA125 serum level of 30 U/mL was found to be best, with 78% sensitivity, 78% specificity, 72.6% false positive rate, and 3.1% false negative rate (Figure 1A). This evidence concerns the gene MUC16 and metastatic malignant neoplasm in the lymph nodes.