Preoperative assessment of lymph node involvement represents a critical step for determining the extent of surgery in patients with endometrial carcinoma, especially in patients with clinical stage I. Interestingly, the mean value of CA125 in the 6.4% patients was significantly higher than those with FIGO stage I. In the current study, the CA125 serum level of 25 U/mL was the best cutoff to determine the lymph node metastasis without influence of adnexal involvement, distant metastasis and positive peritoneal cytology. The gene discussed is MUC16; the disease is endometrial carcinoma.