G3BP1 and breast carcinoma: To determine whether G3BP1 affected tumor growth and lung metastasis in 4T1 cells in vivo, we constructed two stably G3BP1 shRNA-expressing clones (G3BP1(−)-1 and G3BP1(−)-2) by transfecting the pGU6/hygro-mus-shG3BP1 or pGU6/hygro-control vector into murine mammary carcinoma 4T1-luc cells.