However, in the multivariate analysis, the Cox proportional hazards model including Cyclin B1 expression, tumor size, lymph node metastasis, and distant metastasis, identified tumor size, lymph node metastasis and distant metastasis as the independent prognostic indicators for the overall survival rate of patients with colorectal cancer (HR:0.564, P = 0.022; HR:0.205, P = 2.0×10–8; HR: 0.148, P = 1.0×10–6, respectively), but not Cyclin B1 expression (p = 0.45) (Fig 2D). This evidence concerns the gene CCNB1 and metastatic malignant neoplasm in the lymph nodes.