CCNB1 and colorectal cancer: However, in the multivariate analysis, the Cox proportional hazards model including Cyclin B1 expression, tumor size, lymph node metastasis, and distant metastasis, identified tumor size, lymph node metastasis and distant metastasis as the independent prognostic indicators for the overall survival rate of patients with colorectal cancer (HR:0.564, P = 0.022; HR:0.205, P = 2.0×10–8; HR: 0.148, P = 1.0×10–6, respectively), but not Cyclin B1 expression (p = 0.45) (Fig 2D).