Cyclin B1 protein was overexpressed in colorectal cancer tissues without metastasis, compare to the normal colorectal tissues (P = 5.51×10–5), whereas the Cyclin B1 protein level significantly decreased in colorectal cancer tissues with metastasis, when compared to those without metastasis (P = 0.012) (Fig 1E). This evidence concerns the gene CCNB1 and colorectal cancer.