CD34 and cytomegalovirus infection: Unfortunately, primary CD34+ cells are notoriously difficult to manipulate by transfection, so we turned to the CD34+ cell line Kasumi-3, which is easily transfectable (Amaxa, Lonza; all transfections had an efficiency of >95 %, demonstrated with green fluorescent control) and reproduces many of the major aspects of latent HCMV infection.