Our data indicated that digoxin suppresses FAK-Src, paxillin, PI3K/AKT, MEK/ERK, STAT3 and p130Cas-JNK signaling pathways in various EGFR-containing lung cancer cells, suggesting that digoxin inhibits cancer cell invasion by decreasing Src activation and the activation of related signaling pathways. This evidence concerns the gene PIK3CA and lung carcinoma.