For HFE mutations (C282Y, H63D and S65C), associated with an iron overload in homozygous or compound genotypes characteristic of HH, we observed a higher frequency (20.3% heterozygous and 1.6% homozygous) of H63D, consistent with previous studies reported internationally [21] and in a Brazilian population of blood donors from São Paulo [41]. This evidence concerns the gene HFE and Tangier disease.