To validate the microarray results, we performed real-time RT-PCR, which showed that the EZH2 expression level decreased 0.4-fold in CD34+/CD38− AML cells following shRNA-mediated downregulation of CD82 expression (n = 7; Fig 1A and 1B) [30, 31]. The gene discussed is CD82; the disease is acute myeloid leukemia.