EHF and esophageal squamous cell carcinoma: Surprisingly, the overexpressed ESE3 from the three different plasmids was localized in the nuclei of both transiently and stably transfected EC9706 and KYSE150 cells, which was different to the cytoplasmic localization of endogenous ESE3 in ESCC cells (Figs 4, 5 and 6).