While the ND mice exhibit a diffused dispersion of intracellular MG53, illustrated by the uniform redness of their muscle fibers (Fig 4B, top middle panel), the HFD-fed mice display a punctated distribution of intra-fibrillar MG53, indicated by the small red clumps scattered throughout the TA muscle fibers of the mice with metabolic syndrome (Fig 4B, bottom middle panel). The gene discussed is TRIM72; the disease is metabolic syndrome.