To examine whether thioridazine regulates autophagy flux in GBM cells, immunofluorescence assay was used to analyze the colocalization of LAMP-2 (lysosome-associated membrane protein 2; marker of late endosomes/lysosomes) and LC3 in GBM cells after a 24-h thioridazine treatment. This evidence concerns the gene MAP1LC3A and glioblastoma.