It has been shown that GBM cells response better to agents that induce autophagy than apoptosis.18, 19, 20 To investigate whether the mechanisms of thioridazine undergo autophagy, apoptosis or both pathways in GBM cells, we used western blot analysis to examine the level of LC3-II (a marker of autophagy), cleaved-Caspase-3 (c-Caspase-3) and cleaved-PARP (c-PARP; markers of apoptosis). This evidence concerns the gene CASP3 and glioblastoma.