In the prophylactic study, five successive combined treatments with CnB and IFN-γ before inoculation with B16-F10 melanoma cells significantly prolonged the survival of mice compared with treatment with normal saline (P<0.001), CnB alone (P<0.05), or IFN-γ alone (P<0.01) (Figure 7e). The gene discussed is PPP3R1; the disease is melanoma.