In supernatants of HPV-positive tumors upon PMA and ionomycin stimulation we detected higher levels of IL-10, IL-17, IL-21, TNFα and IFNγ compared to supernatants of HPV-negative tumor samples; however, only the levels of IL-17 showed statistically significant differences (p = 0.030) (Fig. 4C). This evidence concerns the gene TNF and neoplasm.