As a complementary approach to screen for pathways that interact with EGFR and FGFR to drive growth and thereby effect intrinsic sensitivity to TKIs, HNSCC cells were transduced with a genome-wide shRNA library (UMSCC25) or a kinome-targeting shRNA library (UMSCC25 and UMSCC8) and selected for resistance to puromycin as described in the Materials and Methods. This evidence concerns the gene EGFR and head and neck squamous cell carcinoma.