In order to determine the relative suppressive capacity of CXCR3+ ICOS+ Treg cells in our T1D transfer model, we reconstituted NOD.TCRα-/- mice with FACS-sorted Teff cells alone or co-transferred with either CXCR3+ICOS+ Treg cells or CXCR3-ICOS+ Treg cells. The gene discussed is ICOS; the disease is type 1 diabetes mellitus.