Renal function and histomorphology were assessed along with levels ofH2S, cystathionine-γ-lyase (CSE), Toll-like receptor 4 (TLR4),interleukin (IL)-10, IL-12, and tumor necrosis factor (TNF)-α in renal tissue.Hemorrhagic shock induced AKI with increased urea and creatinine levels in plasma andhigher H2S, CSE, TLR4, IL-10, IL-12, and TNF-α levels in renal tissue.PHSML drainage significantly reduced urea, creatinine, H2S, CSE, and TNF-αbut not TLR4, IL-10, or IL-12. This evidence concerns the gene TLR4 and acute kidney injury.