Other genes involved in one-carbon metabolism were found transcriptionally repressed in HCC tissue, although the methylation pattern was unchanged in BHMT1, BHMT2, CBS, GNMT, and MTHFD2L in cancer as compared to cancer-free tissue (Additional file 3: Table S3) or decreased in FOLH1 (Table 5). The gene discussed is MTHFD2L; the disease is hepatocellular carcinoma.