AFP and hepatocellular carcinoma: Location of AFP was in cytoplasm and the location of Src both in cytoplasm and cytoblast of the cells, these protein expressed much higher level in HCC tissues than in other liver tissues (Figure 1A); Western blotting detection showed that expression of AFPR emerged in HBV-infected liver tissues, but Src and AFP expression were limited to cirrhotic and HCC tissues (Figure 1B); Moreover, the expression of AFPR and AFP significantly higher than Src in hepatitis tissues (Additional file 1 and Figure 1B).