In this investigation, the results indicated that human normal liver cells, L-02 were transfected with HBx-expressed vectors been able to stimulate expression of AFPR priors to the expression of AFP and Src, the results implicated that AFP played important role in inducing malignant transformation of liver cells and enhancing HCC cells malignant behavior was mediated by AFPR. Here, AFP is linked to hepatocellular carcinoma.