Previously, we have found that AFP enhanced proliferation of HCC cells was mediated by AFPR, AFPR was identified as G-protein combined receptor, AFPR signal mediated cAMP and [Ca2+]i transduction of receptor signal to promoted expression of N-Ras and c-myc [19], these results implied that AFPR signal was also a critical factor for HCC development. Here, NRAS is linked to hepatocellular carcinoma.