Recently, documents reported that AFP played pivotal role in HCC development and malignant behavior of liver cells [29-31], cytoplasmic AFP activated PI3K/AKT signal to stimulate expression of Ras, CXCR4 and Src through inhibiting activity of PTEN [16,21], AFP also inhibited the PI3K/AKT pathway through promoting ubiquitination of PTEN to stimulated malignant phenotype of HCC cells [32]. The gene discussed is CXCR4; the disease is hepatocellular carcinoma.