Replication using an independent sample set with n = 13–15, revealed similar results: There were significant increases in the mRNA levels of RhoA (1.35-fold, p = 0.0289), ROCK1 (1.32-fold, p = 0.0296), PRK2 (1.26-fold, p = 0.0378), Profilin1 (1.47-fold, p = 0.0202), Cofilin1 (1.63-fold, p = 0.0129), and MYPT1 (1.23-fold, p = 0.0306) as shown in Fig. 1a, suggesting significant modulation of the Rho kinase pathway in human HD leukocytes. Here, PKN2 is linked to Huntington disease.