We then applied the interruption approach using miRNA-301a inhibitor treated PCa cells and results revealed that it could partially reverse pre-adipocytes capacity to promote PCa invasion (Fig. 4C), and importantly, addition of miRNA-301a to the PCa cells also show the similar modulation of the AR/TGF-β1/Smad/MMP-9 signals as pre-adipocytes co-cultured with PCa cells (Fig. 4D). This evidence concerns the gene AR and posterior cortical atrophy.