In summary, our results conclude that infiltrating pre-adipocytes could promote PCa metastasis via modulation of the miR-301a/AR/TGF-β1/Smad/MMP9 signaling (Fig. 6), which might explain the link of potential obesity influences on the PCa progression. This evidence concerns the gene TGFB1 and obesity due to melanocortin 4 receptor deficiency.