To further validate the regulatory role of miR-199a-5p on the expression of TGFBR1 and JunB, we transfected hsa-mir-199a-5p mimics into the human kidney carcinoma 786-O cells, which express high levels of TGFBR1 and JunB, and low level of miR-199a-5p as shown in Fig 3. The gene discussed is TGFBR1; the disease is renal carcinoma.