In accordance with the significantly high methylation density in prostate cancer tissues as shown by Wilcoxon Signed-rank test (P value = 0.008, Z = -2.649), a statistically significant overexpression of DNA methyl-transferase-1 (DNMT1) (34%, P value<0.01), de-novo-methyl-transferase (DNMT3b) (50%; P value<0.05) and HIF-1α (44%; P value<0.05) genes were observed in cancer tissues w.r.t. 18S rRNA and ACTB controls (Figs 3D, 4A–4B and S1). This evidence concerns the gene DNMT1 and Familial prostate cancer.