BBC3 and lung cancer: To define a mechanism through which the presence of caffeine caused an increase of cisplatin-induced apoptosis in both HTB182 and CRL5985 lung cancer cells, we determined the expression levels of BAD, Bcl-XL, and PUMA, three important proteins involved in the DNA damage-induced apoptosis process [14], in both untreated and cisplatin/caffeine-treated HTB182 and CRL5985 lung cancer cells (Figure 3).