The major finding of the present study is that nonsense and missense mutations of mitND6 gene are correlated with the clinical features of lung adenocarcinoma and that in adenocarcinoma cell lines (A549 cells) such mutations lead to reduced NADH dehydrogenase activity, increased ROS production and concomitantly promote cell migration and invasion. This evidence concerns the gene ENSG00000254051 and lung adenocarcinoma.