Herein, we investigated in a phase I clinical trial a chemo-immunotherapy combination approach based on IFN-DCs injected into metastatic tumor lesions of patients with advanced melanoma pre-treated one day before with DTIC, with the aim of triggering a strong and long-lasting immune response against melanoma antigens released in situ, as a consequence of the chemotherapy-induced cell death, and possibly taken up by the locally injected IFN-DCs. Here, IFNA1 is linked to melanoma.