These results suggest that LPS preconditioning alters transcription in cells, inhibits the production of TNF-α and other pro-inflammatory factors induced by NF-κB, enhances the level of IFN-β induced by IRF3, alleviates the secondary inflammation after cerebral ischemia injury, and produces ischemic tolerance (Figure 1B). The gene discussed is NFKB1; the disease is Cerebral ischemia.