After 2 h of middle cerebral artery occlusion (MCAO) followed by reperfusion for 22 h, the volume of cerebral infarction in TLR3- or TLR9-deficient mice was not significantly reduced, and there was no improvement in the neurological score [40], suggesting that TLR3 or TLR9 had no protective effect on MCAO-induced ischemic cerebral damage. This evidence concerns the gene TLR9 and cerebral infarction.