We performed Quantitative Real-time PCR (qPCR) on 6 up-regulated genes (COL1A, BGN, SPP1, MELK, IGFBP4, SPARC) and 4 down-regulated genes (PGC, SST, MT1X, S100P) to verify our data in gastric cancer tissues (Tumor) and noncancerous tissues (Normal). The gene discussed is SPP1; the disease is neoplasm.