The expression levels of three selected deregulated genes, CXCR3, IDO-1, and IFNG were confirmed by quantitative RT-PCR from five cases and normalized to reference gene, GAPDH. The relative mRNA expression levels were then compared with the levels in reference tissue (erythema nodosum, EN) and results were presented as fold changes. This evidence concerns the gene IDO1 and Erythema nodosum.