To further assess the effects of miR-221 on TGFβ1-induced EMT, we transfected control and miR-221 inhibitor into T24 and RT4 cells, and then cells were treated with TGFβ1, and detected the effects of miR-221 on TGFβ1-induced EMT in bladder cancer cells. The gene discussed is TGFB1; the disease is urinary bladder cancer.