Because EMT is of particular significance as a marker of tumor invasion and metastasis and TGFβ1 treatment represents a classical induction approach for in vitro EMT research, we believe that elaborating both the specific roles of miR-221 in TGFβ1-induced EMT models of bladder cancer and the latent molecular mechanisms will enlarge our theoretical understanding of human bladder cancer and provide future clinical approaches to treating this disease. Here, TGFB1 is linked to neoplasm.