CTSG and neoplasm: In particular, ER-negative tumours showed significantly higher beta methylation values than ER-positive tumours at the three cg loci located within the first intron (cg04063345, cg15626350, cg00601836; p < 1.0 × 10−7 for all), which were in the same region as the p2 MVR assayed by pyrosequencing.