NES and Stroke: Indeed, when stained with shh and nestin antibodies in post-stroke brain, we identified a number of cells in the SVZ in wt mice that are nestin+/shh+ (Fig 6II panel A, arrows) but only a few cells (Fig 6II panel B, arrow) in shh iKO animals to be shh positive, suggesting deletion of the shh gene in the SVZ niche.