CCN1 and cancer: This observation suggested that the tCCN1-Prom could serve as a cancer-selective prom for making cancer-selective oncolytic Ads and potentially CTVs. To monitor promoter activity quantitatively we cloned the 5′-flanking promoter region of the tCCN1-Prom (approximately 830-bp of the full length CCN1-Prom), inserted it upstream of a luciferase reporter gene and evaluated cancer-selective activity of this tCCN1-Prom in CaP cell lines.