EGFR and lung adenocarcinoma: To screen for the expression of GAS5 in the NSCLC cell lines, we used qRT-PCR analysis to assess GAS5 expression in the A549 (K-RAS mutant, EGFR wild-type), H1299 (EGFR wild-type, N-RAS), H1975 (T790M EGFR mutant), and HCC827 (EGFR mutant) lung adenocarcinoma cell lines.