In the present study, we examined both the mRNA and protein expression levels of Talin-1 in NPC cell lines and clinical tissue samples, and also analyzed the association between Talin-1 expression and the clinical characteristics of a cohort of patients with NPC from Guangdong Province, an area of China where the population have a high risk of NPC. This evidence concerns the gene TLN1 and nasopharyngeal carcinoma.