After infection for 72 h with MOI 50, compared to control virus Ad/AFP, Ad/AFP-Casp-AFP-amiR inhibited HepG2 cell survival by 56.40% (p < 0.001, n = 5), Hep3B by 5.90% with no significant difference and HL7702 by 8.72% (p < 0.01,n = 5) respectively (Figure 6). This evidence concerns the gene AFP and infection.