After infecting HCC cells for 72 h, Ad/AFP-Casp-AFP-amiR promoted early apoptosis by 35.97% (p < 0.05, n = 4) in HepG2, 41.15% (p < 0.05, n = 4) in Hep3B and 4.66% (p > 0.05, n = 4) in HL7702 respectively by Annexin V staining followed by flow cytometric examination, as shown in Figure 5B. The gene discussed is AFP; the disease is hepatocellular carcinoma.