In our study, a recombinant 1018 bp AFP promoter comprised of the enhancer region from −4120 to −3300 and the basal promoter from −180 to +1 of human AFP gene showed high HCC-specific activity [26], that the specific transcriptional factors/activators could bind to those specific binding sites in AFP enhancer and basal promoter to activate transcription of the downstream genes. Here, AFP is linked to hepatocellular carcinoma.