Quantitative assessment of ICC area was performed by single IF staining using KIT-ir (two different, rabbit and goat, KIT antibodies—Table 3) and PDE3A-ir on adjacent sections of colon for WT, Spry4 KO and KitWT/K641E genotypes (n = 5–7 animals per group). The gene discussed is SPRY4; the disease is intrahepatic cholangiocarcinoma.