Inaddition to its effects on the primary tumor, the reduced proliferative capacityof SIRT7-deficient cancer cells could also contribute to faster growth ofmetastatic lesions, even without fundamental changes in metastatic potential.Therefore, to exclude effects of cell proliferation on scoring of metastaticactivity, we partially depleted SIRT7 to levels at which little or nosignificant changes on primary tumor growth were observed (Fig.6A, B). The gene discussed is SIRT7; the disease is cancer.