Consistent with this impaired migration and invasiveness,staining for F-actin (Phalloidin staining) revealed a collapsed actincytoskeleton upon SIRT7 depletion in PC3 cells (Fig. 2D).Notably, SIRT7 inactivation in the HT1080 sarcoma cell line led to similarreductions in cell migration during wound healing and in Transwell invasion(Fig. 2E, F). The gene discussed is SIRT7; the disease is sarcoma.