In this case, the MB could act not only as detector of the over-expression of the mRNA for the survivin (diagnosis) but also as the blocking agent of the synthesis of the protein itself (therapy), as we demonstrated with Real-time PCR and western blotting experiments which showed a time-dependent reduction of survivin mRNA and protein after 100 nM-MB treatment, respectively, with the molecular beacon transfected into A375 melanoma cells [31,32]. This evidence concerns the gene BIRC5 and melanoma.