Strikingly, infection of HeLa cells revealed that PACS-1 and Nef reconstitute a functional fluorophore when expressed from the pNL4-3 F2A-PACS-1-VN Nef-VC vector, indicating that PACS-1 and Nef are expressed and are in close proximity (Fig 4B, column 3). The gene discussed is S100B; the disease is infection.